Organelle Membranes from Germinating Castor Bean Endosperm
نویسندگان
چکیده
منابع مشابه
Purification of pyruvate kinase from germinating castor bean endosperm.
Cytosolic pyruvate kinase from endosperm of germinating castor beans (Ricinus communis L.; cv Hale) has been purified 3100-fold to apparent homogeneity and a final specific activity of 203 micromole pyruvate produced/minute per milligram protein. Purification steps included: heat treatment, polyethylene glycol fractionation, Q-Sepharose, ADP-agarose, Mono-Q and Phenyl Superose chromatography. N...
متن کاملLipid composition of organelles from germinating castor bean endosperm.
Glyoxysome, endoplasmic reticulum, mitochondria, and proplastid fractions were isolated from endosperm of castor beans (Ricinus communis) germinated for 5 days at 30 C. Samples from sucrose density gradients were diluted with 0.15 m KCI and the membranes pelleted. Lipid extracts of these membranes were analyzed for phosphoglyceride, acyl lipid, and sterol content. The endoplasmic reticulum cont...
متن کاملAccumulation of free ricinoleic Acid in germinating castor bean endosperm.
Lipids from the endosperm of germinating castor bean (Ricinus communis var. Hale) were separated by thin layer chromatography and quantitated by gas chromatography. During the later stages of lipid breakdown (4-6 days germination at 30 C), several lipid classes were found in addition to the storage triglycerides, which are triricinoleins for the most part. One was identified as free ricinoleic ...
متن کاملThe origin and turnover of organelle membranes in castor bean endosperm.
The origin and turnover of organelle membranes in castor bean (Ricinus communis L. var. Hale) endosperm was examined using choline-(14)C as a phospholipid precursor. On sucrose gradients three major particulate fractions were separated; a light membranous fraction (density 1.11-1.13 gram per cm(3)), the mitochondria (1.18 gram per cm(3)), and the glyoxysomes (1.24 gram per cm(3)). Choline-(14)C...
متن کاملPyruvate Dehydrogenase Complex from Germinating Castor Bean
Subceliular organelles from castor bean (Ricuns commnwuis) endosperm were isolated on discontinuous sucrose gradients from germinating seeds which were 1 to 7 days postbit o. Marker enzyme activities of the organeUes were measured (fumarase, catalase, and triose phosphate isomerase) and the homogeneity of the organelle fractions was examined by electron microscopy. Pyrumate dehydrogenase comple...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.67.1.21